ASTM E1611-21

Standard Guide for Conducting Sediment Toxicity Tests with Polychaetous Annelids

ACTIVE

About this standard

Languages
English
Type
ASTM
Status
ACTIVE
Publication date
01 November 2021
Withdrawn Date

About this training

Summary

< div class=" SectionLevel2" > < p class=" subsec1 cdone2" > < span class=" Head3 cdone" > 1.1< / span>  This guide covers procedures for obtaining laboratory data concerning the adverse effects of potentially contaminated sediment, or of a test material added experimentally to contaminated or uncontaminated sediment, on marine or estuarine infaunal polychaetes during 10-day or 20 to 28-day exposures. These procedures are useful for testing the effects of various geochemical characteristics of sediments on marine and estuarine polychaetes and could be used to assess sediment toxicity to other infaunal taxa, although modifications of the procedures appropriate to the test species might be necessary. Procedures for the 10-day static test are described for < span style=" font-style:italic font-weight:normal " class=" overriddenformat" > Neanthes arenaceodentata < / span> and < span style=" font-style:italic font-weight:normal " class=" overriddenformat" > Alitta virens < / span> < span style=" font-style:normal " > < span class=" footnote-link" > 2< / span> < / span> (formerly < span style=" font-style:italic font-weight:normal " class=" overriddenformat" > Nereis virens< / span> and < span style=" font-style:italic font-weight:normal " class=" overriddenformat" > Neanthes virens< / span> ) and for the 20 to 28-day static-renewal sediment toxicity for < span style=" font-style:italic font-weight:normal " class=" overriddenformat" > N. arenaceodentata< / span> .

< / div> < div class=" SectionLevel2" > < p class=" subsec1 cdone2" > < span class=" Head3 cdone" > 1.2< / span>  Modifications of these procedures might be appropriate for other sediment toxicity test procedures, such as flow-through or partial life-cycle tests. The methods outlined in this guide should also be useful for conducting sediment toxicity tests with other aquatic taxa, although modifications might be necessary. Other test organisms might include other species of polychaetes, crustaceans, and bivalves.

< / div> < div class=" SectionLevel2" > < p class=" subsec1 cdone2" > < span class=" Head3 cdone" > 1.3< / span>  Other modifications of these procedures might be appropriate for special needs or circumstances. Although using appropriate procedures is more important than following prescribed procedures, the results of tests conducted using unusual procedures are not likely to be comparable to those of many other tests. Comparisons of the results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting sediment tests with infaunal organisms.

< / div> < div class=" SectionLevel2" > < p class=" subsec1 cdone2" > < span class=" Head3 cdone" > 1.4< / span>  These procedures are applicable to sediments contaminated with most chemicals, either individually or in formulations, commercial products, and known or unknown mixtures. These procedures can be used with appropriate modifications to conduct sediment toxicity tests on factors such as temperature, salinity, dissolved oxygen (DO), and natural sediment characteristics (for example, particle size distribution, organic carbon content, and total solids). These procedures can also be used to conduct bioconcentration tests and in situ tests, and to assess the toxicity of potentially contaminated field sediments, or of materials such as sewage sludge, oils, particulate matter, and solutions of toxicants added to sediments. A median lethal concentration (LC50) or median sublethal effect concentration (EC50) of toxicants or of highly contaminated sediment mixed into uncontaminated sediment can be determined. Materials adhering to sediment particles or dissolved in interstitial water can be tested.

< / div> < div class=" SectionLevel2" > < p class=" subsec1 cdone2" > < span class=" Head3 cdone" > 1.5< / span>  The results of 10-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality relative to a control or reference sediment. The results of 20 to 28-day toxicity tests with contaminated sediments can be reported as a LC50 if a series of concentrations is tested or as a percent mortality or growth relative to a control or reference sediment.

< / div> < div class=" SectionLevel2" > < p class=" subsec1 cdone2" > < span class=" Head3 cdone" > 1.6< / span>  This guide is arranged as follows:

< div class=" ParaCenter" > < table style=" border-collapse: collapse border-top-style:none border-bottom-style:none " class=" datatable gridtype" width=" 462px" > < col align=" left" width=" 370px" > < / col> < col align=" center" width=" 93px" > < / col> < thead class=" cdone" > < tr> < td style=" vertical-align:top " > < p style=" text-align:left " class=" tablecell_standard" >  

< / td> < td style=" vertical-align:top " > < p style=" text-align:center " class=" tablecell_standard" > Section

< / td> < / tr> < / thead> < tbody class=" cdone" > < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Referenced Documents

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 2< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Terminology

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 3< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Summary of Guide

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 4< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Significance and Use

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 5< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Interferences

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 6< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Apparatus

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 7< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Facilities

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 7.1< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Construction Materials

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 7.2< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Test Chambers

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 7.3< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Cleaning

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 7.4< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Acceptability

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 7.5< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Hazards

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 8< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Test Water

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 9< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  General Requirements

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 9.1< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Source

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 9.2< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Preparation

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 9.3< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Characterization

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  < span class=" Body-link1 cdone" > 9.4< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Test and Control Sediments

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  General

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10.1< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Characterization

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10.2< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Control Sediment

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10.3< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Field-Collected Test Sediment

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10.4< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Reference Sediment

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10.5< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Laboratory-Spiked Test Sediment

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10.6< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Test Concentration(s)

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 10.7< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Addition of Toxicant to Sediment

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > 10.8

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > Test Organisms

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 11< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Species

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 11.1< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Age

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 11.2< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Feeding

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 11.3< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Source

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 11.4< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Collection and Handling

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 11.5< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Quality

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 11.6< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Experimental Design

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 12< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Controls

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 12.2< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Field Survey Design

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 12.3< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Laboratory Experiments

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 12.4< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Procedure

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Dissolved Oxygen

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.1< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Temperature

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.2< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Salinity

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.3< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Light

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.4< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Feeding

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.5< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Beginning of Test

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.6< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Duration of Test

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.7< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Biological Data

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.8< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   Other Measurements

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 13.9< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Analytical Methodology

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 14< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Acceptability of Test

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 15< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Interpretation of Results

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 16< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Report

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 17< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Keywords

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > 18< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  Annexes

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >  

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   < span class=" overriddenformat" style=" font-style:italic font-weight:normal " > Neanthes arenaceodentata< / span>

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > Annex A1< / span>

< / td> < / tr> < tr> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" >   < span class=" overriddenformat" style=" font-style:italic font-weight:normal " > Alitta virens< / span>

< / td> < td style=" vertical-align:top " > < p style=" text-align:left" class=" tablecell_indented" > < span class=" Body-link1 cdone" > Annex A2< / span>

< / td> < / tr> < / tbody> < / table> < / div> < / div> < div class=" SectionLevel2" > < p class=" subsec1 cdone2" > < span class=" Head3 cdone" > 1.7< / span>  The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

< / div> < div class=" SectionLevel2" > < p class=" caveat cdone2" > < span class=" Head3 cdone" > 1.8< / span>  This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. < span style=" font-style:normal font-weight:normal " class=" overriddenformat" > Specific hazards statements are given in Section < span class=" Body-link1 cdone" > 8< / span> . < / span>

< / div> < div class=" SectionLevel2" > < p class=" WTOcaveat cdone2" > < span class=" Head3 cdone" > 1.9< / span>  This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organizat