NBN EN 14122:2014

Foodstuffs - Determination of vitamin B1 by high performance liquid chromatography

ACTIVE

About this standard

Languages
German, English and French
Type
NBN
Standards committee
CEN/TC 275
Status
ACTIVE
Publication date
25 July 2014
Replaces
NBN EN 14122:2003, NBN EN 14122/AC:2005
ICS Code
67.050 (General methods of tests and analysis for food products)
Withdrawn Date

About this training

Summary

This European Standard specifies a method for the determination of vitamin B1 in food by high performance liquid chromatography (HPLC) with enzymatic treatment and pre- or post-column derivatization. This method has been validated in two interlaboratory studies. The first study was for the analysis of samples of whole meal flour, milk powder/ spray dried milk, freeze-dried mixed vegetables and freeze-dried pig' s liver ranging from 0,295 mg/ 100 g to 0,807 mg/ 100 g. The second study was for the analysis of samples of tube feeding solution, baby food with vegetables, powdered milk, meal with fruits, yeast, cereal, chocolate powder and food supplement ranging from 0,11 mg/ 100 g to 486 mg/ 100 g. Vitamin B1 is the mass fraction of total thiamin including its phosphorylated derivatives.
For further information on the validation, see Clause 8 and Annex B.