NBN ISO 21115:2020

Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1) (ISO 21115:2019)

ACTIVE

About this standard

Languages
English and French
Type
NBN
Standards committee
NBN/E230
Status
ACTIVE
Publication date
29 July 2020
ICS Code
13.060.70 (Examination of biological properties of water)
Withdrawn Date

About this training

Summary

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24- well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).