Standard NBN ISO 21115:2020

Water quality — Determination of acute toxicity of water samples and chemicals to a fish gill cell line (RTgill-W1) (ISO 21115:2019)
Status
:
Active
Publication date
:
7/2020
Languages
:
EN/FR
ICS
:
13.060.70 Examination of biological properties of water
Summary :
This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykis
s) gill, RTgill-W1. Cells in confluent monolayers in 24- well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).
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